Direct Detection of Plasmid-mediated Antibiotic Resistance in Bloodstream Infection by Pcr Using Wire-guided Droplet Manipulation (wdm)
نویسندگان
چکیده
Bloodstream infection (BSI), an expensive and deadly condition, burdens healthcare. Improved BSI treatment relies on rapid, point-of-care diagnosis of causative species and presence of plasmid-mediated drug resistance. We have designed, fabricated and assembled an apparatus for automated PCR amplification by wire-guided droplet manipulation (WDM). WDM minimizes thermal resistance and exploits convective heat transfer to increase reaction speeds. Molecular partitioning within the water-in-oil droplet enables PCR amplification with whole blood (WB) in situ. The V3 region of the 16s rRNA gene and a representative 4.884 kb plasmid were amplified by WDM from 10% WB. Optics has been designed for real-time detection.
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